The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 05, 1982
Filed:
Mar. 17, 1980
Craig L Bentzen, Onex, CH;
Symphar S.A., Geneva, CH;
Abstract
This invention relates to a separation method comprising the introduction of a sample of serum or plasma into a microcolumn containing a support which has a sulfated polysaccharide, preferably heparin, covalently bound to it so as to remove from said sample the .beta.-lipoproteins by binding thereof to this ligand, followed by washing the microcolumn with a first pH buffered solution and collecting the eluant representing a first fraction containing the .alpha.-lipoprotein portion of the sample. Then the microcolumn is washed wth a second pH buffered solution so as to release the ligand-bound .beta.-lipoproteins and to recover a second fraction containing the .beta.-lipoprotein portion of the sample. Afterwards, it is possible to separately determine the .alpha.- and .beta.-lipoprotein-cholesterol, as well as the respective .alpha./.beta. ratios of phospholipids, triglycerides and various apoproteines, and to calculate the respective ratios .alpha./.beta., all useful as clinical diagnostic indexes and allowing to obtain a complete lipoprotein profiling. The invention also concerns a kit comprising in a transportable box all the implements and reagents duly prepared so as to easily and quickly carry out the above method.