The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 26, 1981
Filed:
Mar. 08, 1979
Applicant:
Inventor:
David S Frank, Rochester, NY (US);
Assignee:
Eastman Kodak Company, Rochester, NY (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; C09K / ; G01N / ;
U.S. Cl.
CPC ...
435-7 ; 2323 / ; 252408 ; 252186 ; 2521 / ; 25230116 ; 422 55 ; 422 56 ; 422 57 ; 422 61 ; 424-7 ; 424-8 ; 424 12 ; 435 14 ; 435 28 ; 435188 ;
Abstract
A new peroxidase assay for substances demonstrating peroxidative activity is described which is based on the peroxidase catalyzed oxidation of dichlorofluorescin to dichlorofluorescein by peroxide. The dichlorofluorescin is formed from diacetyldichlorofluorescin either by the direct addition of peroxide or by the inclusion of a peroxide generating system (e.g., glucose plus glucose oxidase or uric acid and uricase) in the reaction mixture. As long as all reactants other than the peroxidase are present in excess, the rate of fluorescence increase is linearly related to the amount of peroxidase in the sample under assay.