The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 24, 1981
Filed:
Apr. 25, 1979
James H Pert, Glenmont, NY (US);
George Dayian, Albany, NY (US);
Hedbergska Stiftelsen, Stockholm, SE;
Abstract
An inexpensive, statically controlled cooling rate device comprising a cassette of metal plates and cardboard insulation in a plastic bag was used to freeze platelets in liquid nitrogen with glycerol-glucose as cryoprotectant. Depending on the thermocouple location, the cooling rate in the freezing bag varied between 22.1.degree. and 38.6.degree. C. per minute, averaging 33.6.degree..+-.1.1.degree. C. per minute at the center. The post-thaw recovery of platelets frozen with this device and reconstituted in plasma averaged 88.6.+-.11.7 percent, compared to 86.1.+-.9.9 percent for nonfrozen but identically processed platelets. [.sup.14 C]Serotonin uptake after 0.5-hour incubation was 95.9.+-.1.9 percent for fresh platelets in platelet-rich plasma, 92.7.+-.4.4 percent for nonfrozen processed platelets, and 81.4.+-.11.8 percent for frozen platelets, increasing to 85.9.+-.7.7 percent after one-hour incubation.