The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 30, 1980
Filed:
Apr. 27, 1979
Joseph L Melnick, Houston, TX (US);
Craig Wallis, Houston, TX (US);
Other;
Abstract
A bacterial staining composition and methods of analysis of both gram-negative and gram-positive bacteria are disclosed. The composition comprises a chelating agent and a basic dye, both of which are operative at a pH above about 7.0. Bacterial staining may be effected by contacting either concentrated or fluidly suspended bacteria with the composition at a neutral or basic pH. Bacteria stained with the composition and concentrated by filtration, centrifugation or the like are readily visible and their presence in a specimen may, thus, be rapidly detected. The gradations of color of the stained, concentrated bacteria correspond to the number of bacteria and semi-quantitative analysis of the bacteria may be effected by comparison with a standard. Differentiation of gram-negative and gram-positive bacteria may be accomplished by treating the stained bacteria with an organic acid wash having a pH of about 2.5 to 2.6. Such a wash completely decolorizes only stained gram-positive bacteria. Finally, a method for determining bacterial susceptibility to antimicrobial agents is provided which comprises incubating bacteria with an antimicrobial agent, staining the bacteria and thereafter comparing the color gradation of the stained, concentrated bacteria with a control or standard.