The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 15, 1980

Filed:

May. 18, 1978
Applicant:
Inventors:

Yuji Nonaka, Shin-nanyo, JP;

Kiyotaka Oyama, Shin-nanyo, JP;

Heijiro Satoh, Shin-nanyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C07G / ;
U.S. Cl.
CPC ...
435212 ; 435213 ; 435218 ; 435219 ; 435225 ; 435 70 ; 435816 ;
Abstract

Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from an organic solvent suspension or dissolving said addition compound by adding a water immiscible organic solvent and separating the organic solvent phase by a liquid-liquid separation. The addition compound can be dissolved in an organic solvent after separating the precipitate from the reaction mixture. The addition compound can be also dissolved by adding a water-immiscible organic solvent to the reaction mixture and the organic solvent phase can be separated from the aqueous phase.


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