The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 18, 1980
Filed:
Feb. 24, 1978
David S Frank, Rochester, NY (US);
Ignazio S Ponticello, Rochester, NY (US);
Eastman Kodak Company, Rochester, NY (US);
Abstract
Methods, compositions and elements for detecting a nitrogen-containing compound capable of releasing ammonia upon enzymatic action are described. The method comprises contacting in an aqueous medium a sample suspected of containing such a compound and a novel assay composition comprising at least one enzyme which catalyzes the decomposition of the compound to ammonia and detecting the ammonia by condensation with a .beta.-diketone in the presence of an aldehyde, preferably formaldehyde, and measuring the color or fluorescence produced by any resulting dihydropyridine. According to a preferred embodiment, the aldehyde is generated in situ and the .beta.-diketone is polymeric. The foregoing assay composition can be incorporated into single-layer or multilayer analytical elements of the type known in the prior art. A preferred such element comprises an isotropically porous spreading layer in fluid contact with a reagent layer. The spreading layer is most preferably non-fibrous.