The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 27, 1979

Filed:

Jan. 30, 1974
Applicant:
Inventor:

Marcos Y Kleinerman, Webster, MA (US);

Assignee:

Block Engineering, Inc., Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ;
U.S. Cl.
CPC ...
424-3 ; 8 1 / ; 8-3 ; 2323 / ; 250302 ; 25230116 ; 252408 ; 424-7 ; 424-8 ;
Abstract

A novel composition of matter, process and apparatus is provided for distinguishing and absolutely counting leukocytes, erythrocytes, reticulocytes, and other cells as well as differently counting and classifying the different types of leukocytes. The composition of matter comprises a plurality of compatible dyes for uniquely distinguishing leukocytes, erythrocytes, reticulocytes, and other cells randomly distributed in a medium. At least one of the dyes imparts a characteristic fluorescence to all leukocytes stained therewith. The nature of the dye composition is such that when the stained leukocytes are irradiated with radiation having the characteristic absorption wavelengths of each dye, the relative intensities of the light emitted and/or transmitted by each leukocyte type defines that particular leukocyte type. Apparatus is therefore provided for irradiating the medium with the characteristic radiation and measuring the radiation emitted by the irradiated leukocytes in each of their characteristic wavelength regions.


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