The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 09, 1979
Filed:
Aug. 01, 1977
Applicant:
Inventors:
Russell C Johnson, Roseville, MN (US);
Russell F Bey, Roseville, MN (US);
Assignee:
The Regents of the University of Minnesota, Minneapolis, MN (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12K / ;
U.S. Cl.
CPC ...
195100 ; 195102 ; 2603474 ; 195 96 ;
Abstract
A process for the preparation of a special protein-free nutrient medium having unexpected efficiency in the propagation of Leptospira. The process comprises strategically admixing a modified or detoxified polysorbate fatty acid to a preformed mixture containing vitamin B.sub.12, vitamin B.sub.1, soluble manganese, magnesium and ammonium salts, and a buffer, in a water diluent adjusted to pH 6.5 to 8.5. The polysorbate fatty acid is modified or detoxified by admixture with activated charcoal followed by settling, decantation, centrifugation and filtration to remove all charcoal particles.