The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 22, 1978
Filed:
Mar. 04, 1977
Max G Reese, Salt Lake City, UT (US);
Becton, Dickinson and Company, East Rutherford, NJ (US);
Abstract
Automated radioimmunoassay directly from serum, for those antigens present in serum in relatively small amounts, is accomplished by use of an immobilized immunoadsorbent having an excess of antibodies, specific to the antigen, covalently bonded to a particulate substrate. The serum is diluted and preincubated in the presence of a radioactive labelled antigen and an antibody specific to the antigen. The preincubated mixture is flowed through the immobilized immunoadsorbent and the labelled and unlabelled antigen not bound to the antibody in preincubation are bound to the immobilized immunoadsorbent while the labelled and unlabelled antigen bound to the antibody during preincubation flow through the immunoadsorbent. An eluting solution is flowed through the immunoadsorbent to release the antigens bound thereto for reuse of the immunoadsorbent. By counting the radioactivity of one or both the fractions which flow through the immunoadsorbent or which is released therefrom, the quantity of a specific antigen in the serum may be assayed.