The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 04, 1977
Filed:
Dec. 11, 1975
Applicant:
Inventors:
Prakash S Masurekar, Webster, NY (US);
Charles T Goodhue, Rochester, NY (US);
Assignee:
Eastman Kodak Company, Rochester, NY (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12D / ; C07G / ; C12B / ;
U.S. Cl.
CPC ...
195 / ; 195 62 ; 195 65 ; 195114 ;
Abstract
An improved fermentation method comprises growing Nocardia cholesterolicum in a medium comprising a carbon source, such as glycerol, yeast extract, a nonionic surfactant, an auxiliary carbon source which is also an inducer of cholesterol oxidase, and trace salts. Yields of cholesterol oxidase up to about 900 U. (international units) per liter have been obtained using the improved method of the present invention in contrast with the yields of 1-3 U. per liter obtained in the prior art. Proper selection of environmental conditions also enables the coproduction of cholesterol esterase.