The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 23, 1977

Filed:

Oct. 01, 1975
Applicant:
Inventors:

Sidney Alan Barker, Birmingham, EN;

Charles John Gray, Birmingham, EN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07G / ;
U.S. Cl.
CPC ...
195 63 ; 195 68 ; 195D / ; 2601 / ;
Abstract

The invention relates to biologically active protein molecules, such as enzymes, attached to water-insoluble solids to render the molecules insoluble. In one aspect the attachment is effected by molecules of diazotized m-diamino-benzene adsorbed on a surface of the solid and chemically linked to the protein molecules. This invention also provides methods for reactivating inactivated biologically active material characterized by a water-insoluble solid with a surface to which has been adsorbed molecules of a diazotized aromatic amine chemically bonded to biologically active protein molecules. One method comprises a) adsorbing additional molecules of a diazotized aromatic diamine on said surface, and b) attaching fresh biologically active protein molecules to said additional molecules of diazotized aromatic diamines. Another method comprises a) treating said material with sodium dithionite to regenerate amine groups, and b) diazotizing said amine groups, and c) attaching thereto biologically active protein molecules.


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