The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 02, 1977
Filed:
Sep. 27, 1976
Prakash G Kadkade, Marlboro, MA (US);
GTE Laboratories Incorporated, Waltham, MA (US);
Abstract
A tissue culture technique and a culture medium used therewith for asexually propagating a plurality of plants of the family Cruciferae are disclosed. According to one aspect of the invention, an excised portion of a donor plant is placed in a culture medium comprising organic and inorganic salts and a preferable concentration of 1 mg/l of Indoleacetic Acid, 0.5 mg/l of Kinetin and 40 mg/l of Adenine Sulfate. The medium induces the simultaneous initiation of buds and roots by the explant to form a plurality of plantlets which may be transplanted into soil for the timely initiation of food and medicinal crops. In accordance with a second aspect of the invention, the pre-culture storage of the requisite plant part in a convenient, disease-free, and pest-free manner is disclosed whereby callus from the excised portion of the donor plant is maintained on a first medium for subsequent transfer to the medium described above. The callus is initiated and maintained, for periods in the order of a year or more, on a medium containing organic and inorganic salts and preferable concentrations of 1.86 mg/l of naphthaleneacetic acid and 2.25 mg/l of N.sub.6 -Benzyladenine.