The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 08, 1977

Filed:

Nov. 17, 1975
Applicant:
Inventors:

Lawrence E Gravely, Louisville, KY (US);

Vernon L Geiss, Floyd Knobs, IN (US);

Richard P Newton, Louisville, KY (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12B / ;
U.S. Cl.
CPC ...
195 96 ; 195-2 ; 195 / ; 131141 ;
Abstract

A process is disclosed for maximizing the growth and nicotine degrading activity of microorganisms effective to degrade nicotine through a biochemical mechanism in which 3-succinoylpyridine is formed. The process involves inoculating a nicotine-containing growth medium with the microorganisms and subjecting the broth to aeration and agitation. During the growth period, the broth should be maintained at a pH of between about 6 and 7.8 and at a temperature between about 10.degree. C and about 45.degree. C. The broth should have an initial nicotine concentration of at least 0.5 mg. per ml. up to an amount which is toxic to the microorganisms.


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