The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 30, 1976

Filed:

Dec. 05, 1975
Applicant:
Inventors:

Kiyochika Tokuyasu, Higashiyamato, JA;

Takeshi Yokobori, Sayama, JA;

Chizuko Nakahara, Hino, JA;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07G / ; A61K / ;
U.S. Cl.
CPC ...
195 / ; 424110 ;
Abstract

A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.


Find Patent Forward Citations

Tokuyasu, Kiyochika. (1976). Methods for extracting and purifying kallidinogenase (U.S. Patent No. 3994782). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/3994782

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