The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 01, 1976
Filed:
Mar. 19, 1974
Jerker Olof Porath, Uppsala, SW;
Jan Gunnar Rosengren, Uppsala, SW;
Exploaterings Aktiebolaget T.B.F., Uppsala, SW;
Abstract
The invention concerns a gel product for chromatography based on agarose, and a method of using the product for hydrophobe salting out adsorption. The gel product is characterized in that the hydrophobe groups are bonded to the agarose with ether or esther binding, and that the hydrophobe groups can be composed of aliphatic hydrocarbons, halogen or hydroxy-substituted alkyl groups, with 1 to 20 carbon atoms, or contain 1 or more, up to 5, aromatic nuclei, unsubstituted or substituted with alkyl, alkyloxy, halogen or NO.sub.2 or have the formula ##EQU1## where R can be alkyl, alkenyl, aryl, aralkyl, aralkoxy, alkaryl, optionally substituted with hydroxy, halogen or nitro, and where the alkyl parts of R contain 1 to 20 carbon atoms. The method of separating the amphipatic substances is based on the fact that they are adsorbed by an amphipatic gel in which the salt concentration in the solution is adjusted beyond the level at which the ion concentration least corresponds to the ion concentration in an aqeous solution of 1 M NaCl, and desorption takes place through lowering the ion concentration and/or lowering the pH and/or lowering the polarity of the solvent. The amphipatic substances are primarily proteins or peptides.