The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 18, 1976

Filed:

Sep. 30, 1974
Applicant:
Inventors:

Donald P Kronish, Rockaway, NJ (US);

William D Young, Montclair, NJ (US);

Assignee:

Warner-Lambert Company, Morris Plains, NJ (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12K / ; C12K / ;
U.S. Cl.
CPC ...
1951 / ; 195100 ; 195102 ;
Abstract

A diagnostic test system for detecting .beta.-galactosidase producing micro-organisms is prepared by impregnating one zone of a bibulous material with an aqueous acetone solution of an o-nitrophenyl-.beta.-D-galactopyranoside substrate and evaporating to dryness; and impregnating a contiguous and separate zone of the bibulous material with a solution of a microbiologically inert buffer system which will maintain a pH of from about 6.5 to 8.5 and evaporating to dryness. Preferably, the bibulous material contains 1.5 mg of dry o-nitrophenyl-.beta.-D-galactopyranoside and 0.02 millimoles of a buffer which will maintain a pH of 7.4. Optionally, a waterproof barrier zone and an identifying dye zone may be present. In use, the impregnated bibulous material is inserted into a test tube of a saline suspension of unknown organism and incubated for 4 hours. The development of a yellow coloration indicates that .beta.-galactosidase is produced by the organism being tested. The diagnostic test system of this invention is stable for 1 year at 4.degree.C., at room temperature, and at 37.degree.C.


Find Patent Forward Citations

Kronish, Donald. (1976). Detection of beta-galactosidase producing micro-organisms (U.S. Patent No. 3957584). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/3957584

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