The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 30, 1976
Filed:
May. 13, 1974
F Paul Carlson, Seattle, WA (US);
Joseph E Ward, III, St. Paul, MN (US);
The Board of Regents of the University of Washington, Seattle, WA (US);
Abstract
Cells of a given type spread among an ensemble of cells of various types are identified and, if desired, counted. Coherent light is directed toward a monolayer of cells of various types. The light scattered by the monolayer of cells is collected by a lens and the collected light is filtered by an optimum filter located in the focal plane of the lens. The optimum filter is a minimum mean-square error linear filter which suppresses light scattered by cells other than the given type of cell whereby the light scattered by the given type is enhanced. The intensity of the light passed by the optimum filter is related to the number of cells of the given type contained in the monolayer of cells. If desired, a second lens may be used to collect the filtered light and focus it toward an integrating and squaring detector. The optimum filter may be obtained photographically. In one form, a first photographic negative of a monolayer of cells having a high percentage of cells of the given type is made at the focal plane of a transforming lens mounted so as to collect the light scattered by the monolayer; and, developed for a gamma of unity. Thereafter, a second photographic negative is made at the same focal plane of a monolayer of cells containing a normal percentage of cells of the given type using the first negative as a filter. The second negative is developed for a gamma of two. The resulting photographic plate is a realization of the desired optimum (minimum mean-square error linear) filter. Alternatively, a sandwich of two photographic plates, one positive and obtained from a monolayer of cells having a high percentage of cells of the given type and the other negative obtained from a monolayer of cells having a normal percentage of cells of the given type, is constructed to provide a physical realization of the desired optimum filter.