The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 20, 1976
Filed:
Jun. 25, 1975
Peter Edgar Hare, Takoma Park, MD (US);
Durrum Instrument Corporation, Palo Alto, CA (US);
Abstract
A method and apparatus for the detection of primary and secondary amino acids in a known sequence in a liquid sample stream. The amino acids are rendered detectable by reaction with a fluorometric reagent such as fluorescamine. A conversion reagent, e.g., N-chlorosuccinimide, is added to the sample streams only when secondary amine is present. Baseline shift in the fluorometric detection is avoided by adding an inert liquid diluent stream to the sample stream at a predetermined flow rate at all times except when the conversion reagent is added. By maintaining the diluent and N-chlorosuccinimide streams under constant pressure and synchronously switching between streams to coincide with the presence of a primary or secondary amino acid, liquid flow through the fluorometer is maintained at a substantially constant rate throughout the run.