The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 06, 1976
Filed:
Nov. 26, 1973
Paul Maldonado, Chatou, FR;
Jean-Pierre Desmarquest, Paris, FR;
Claude Gaillardin, Paris, FR;
Daniel Binet, Rueil Malmaison, FR;
Institut Francaise du Petrole, des Carburants et Lubrifiants, Paris, FR;
Institut Francais du Petrole, Rueil-Malmaison, FR;
Abstract
A process for the preparation of a stable diploid of Candida lipolytica which comprises (a) in a first stage, separately cultivating two haploid strains of Candida lipolytica of opposed sign in a medium rich in assimilable carbon nutrient; (b) in a second stage, cultivating the two resultant strains together in a medium poor in assimilable carbon nutrient; and (c) in a third stage, after the appearance of diploid colonies, treating them with a mutagenic agent to stabilize them. The invention also comprises a process for the preparation of .alpha.-ketoglutaric acid which comprises cultivating a diploid strain obtained by crossing two Candida lipolytica haploid strains of opposite sign in a medium containing, as source of assimilable carbon, at least one hydrocarbon.