Generating adjusted test results from molecular barcoded cell-free dna sequence data

Abstract

The present disclosure provides methods comprising: (a) ligating molecular barcodes to a plurality of cell-free DNA (cfDNA) molecules, wherein the plurality of cfDNA molecules are from a first cell-free biological sample from a subject; (b) ligating molecular barcodes to a second plurality of cfDNA molecules, wherein the second plurality of cfDNA molecules are from a second cell-free biological sample from the subject; (c) generating genetic data using a genetic analyzer, wherein the genetic data relates to genetic information of the subject; (d) receiving the genetic data from the genetic analyzer; (e) comparing the genetic data from the first cell-free biological sample to the genetic data from the second cell-free biological sample to produce an adjusted test result, wherein the adjusted test result is adjusted based upon whether the genetic data from the first cell-free biological sample corroborates or conflicts with the genetic data from the second cell-free biological sample and (f) outputting the adjusted test result into computer memory. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.