Cell repair protein extract and preparation method therefor and use thereof

Abstract

The present disclosure relates to a cell protein extract having cell repair effect. The preparation thereof includes the following steps: (1) placing mesenchymal passage cells with a density of 1.0×10cells/mL to 5.0×10cells/mL in a culture medium containing DMEM/F12 40-50%, RPMI1640 40-50%, bovine serum albumin (BSA) 0.1-2%, epidermal growth factor (EGF) 1-15 μg/mL, fibroblast growth factor (FGF) 1-15 μg/mL, insulin transferrin 1-15 μg/mL, compound amino acids (18AA) 0.01-0.1%, and 2-10 μmol/L of a stressor, and then culturing the cells under conditions of 37.0° C.±0.5° C. and 5%±1.0% COfor 10 minutes to 14 hours, and then performing isolation, washing, and collecting cells, wherein the stressor is selected from any one of compounds 1-16 or a combination thereof; (2) performing an ultrasonic treatment on the collected cells at 2° C.-8° C. to prepare a cell lysate, and filtering same so as to obtain the cell protein extract.