Methods and systems for adjusting a training gate to accommodate flow cytometer data

Abstract

Methods for adjusting a training gate prepared from a first set of flow cytometer data to accommodate a second set of flow cytometer data are provided. In embodiments, methods include generating an image for each of the first and second sets of flow cytometer data. In some instances, generating an image includes organizing the data into two-dimensional bins, and assigning shades to each bin such that the bins are represented by pixels. In some instances, methods include warping with a computer implemented algorithm the generated image of the first set of flow cytometer data such that it maximizes resemblance to the second set of flow cytometer data, and applying the same transformation to the training gate. In some embodiments, methods include overlaying the adjusted training gate onto the generated image of the second set of flow cytometer data. Systems and computer-readable media for adjusting a training gate are also provided.