Calibration and profiling of a nanopore array device

Abstract

A method of calibrating a nanopore array device. The nanopore array device comprising: a common electrode, an array of sensing elements each comprising a sensing electrode and a nanopore channel, and an ionic solution in contact with the common electrode and the array of sensing electrodes, the ionic solution providing electrical communication between the common electrode and each of the array of sensing electrodes via the corresponding array of nanopore channels. The method comprises the steps of: applying a two or more test signals across the nanopore channels between the common electrode and array of sensing electrode measuring a corresponding current or voltage value associated with each sensing electrode for each test signal, determining an offset value for each sensing electrode from the measured current or voltage signals, and calculating a general offset value from the determined offset values, and applying a calibrated signal between the common electrode and sensing electrodes; wherein the calibrated signal is adjusted by the general offset value.