The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 15, 2026

Filed:

Nov. 23, 2022
Applicant:

10x Genomics, Inc., Pleasanton, CA (US);

Inventors:

Zahra Kamila Belhocine, Pleasanton, CA (US);

Justin Costa, Union City, CA (US);

Eswar Prasad Ramachandran Iyer, Sunnyvale, CA (US);

Assignee:

10X GENOMICS, INC., Pleasanton, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/682 (2018.01); C12Q 1/6841 (2018.01); C12Q 1/6844 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6841 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6844 (2013.01);
Abstract

The present disclosure relates in some aspects to methods and compositions for analyzing a target nucleic acid, such as in situ detection of a region of interest in a polynucleotide in a cell or tissue sample. In some aspects, provided herein are circular probes (e.g., dumbbell probes) for analyzing a target nucleic acid, as well as methods comprising an enzymatic treatment to de-circularize unbound or non-specifically bound circular probes. Circular probes specifically bound to target nucleic acids remain intact during and after the enzymatic treatment and can be detected, e.g., via rolling circle amplification (RCA) of the circular probes and detection of the RCA products.


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