The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 01, 2026

Filed:

Jan. 09, 2024
Applicant:

Agilent Technologies, Inc., Santa Clara, CA (US);

Inventors:

Jason Epps, Erie, CO (US);

Joseph Guiles, Broomfield, CO (US);

Todd B Kreutzian, Golden, CO (US);

Steve Bunch, Berthoud, CO (US);

Assignee:

Agilent Technologies, Inc., Santa Clara, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B01J 19/0046 (2013.01); B01L 3/502715 (2013.01); C12Q 1/6806 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0627 (2013.01);
Abstract

Methods and systems for synthesizing an oligonucleotide in a reaction chamber comprising a solid support. A nucleotide monomer is selected for addition to an oligonucleotide. An inlet stream comprising the selected monomer is fed to the reaction chamber (column) of the reaction chamber and conditions are provided in the reaction chamber for oligonucleotide synthesis. An outlet stream from the reaction chamber can be split and directed to the waste drain and/or to an analysis station where the eluent is analyzed by mass spectrometry (MS) to confirm the identity of the monomer fed through the inlet to the reaction chamber. Based on results from analyzing by MS, it is determined whether the outlet stream comprises the selected nucleotide monomer or a derivative thereof.


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