Single-cell screening device, screening assembly, screening method, and use thereof

Abstract

A single-cell screening device, a screening assembly, a screening method, and use thereof. The single-cell screening device includes a body, a plurality of spray holes, heating portions, and a flow channel. The spray holes are located in the body, the heating portions are located in the spray holes and used for heating a liquid entering the spray holes to generate bubbles so as to spray out cells. The flow channel is located in the body and extends to the spray holes according to a preset path, so as to deliver a cell suspension into the spray holes. The single-cell screening device of the present disclosure has a simple design and is easy to fabricate. Cell printing (spraying) using the screening assembly of the present disclosure has a much higher cell printing speed than conventional methods, allowing for a rapid cell sorting process.