The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 25, 2026

Filed:

May. 31, 2017
Applicant:

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Paulo Andres Garcia Dominguez, Cambridge, MA (US);

Zhifei Ge, Cambridge, MA (US);

Rameech N. Mccormack, Cambridge, MA (US);

Cullen Richard Buie, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 13/00 (2006.01); B01L 3/02 (2006.01); C12M 1/42 (2006.01);
U.S. Cl.
CPC ...
C12N 13/00 (2013.01); B01L 3/0275 (2013.01); C12M 35/02 (2013.01); B01L 2300/0832 (2013.01); B01L 2400/0415 (2013.01);
Abstract

Methods and apparatuses for cell electroporation are provided. An apparatus includes a fluid transport structure, such as a pipette, that includes an electroporation structure defining a flow path having a constriction. The fluid transport structure also includes at least two conductive elements configured to produce an electric field in the flow path. The conductive elements are in operative arrangement with each other and are configured to expose cells contained within a cell suspension flowing through the fluid transport structure to an electric field that is sufficient to electroporate at least a subset of the cells in the flow path.


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