The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 04, 2026

Filed:

Dec. 13, 2022
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Mark J. Schnitzer, Stanford, CA (US);

Radosław Chrapkiewicz, Sunnyvale, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G01J 3/02 (2006.01); G01J 3/08 (2006.01); G01J 3/10 (2006.01); G01J 3/44 (2006.01); G01N 21/63 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); G02B 21/18 (2006.01); G02B 21/36 (2006.01); G02B 27/10 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6458 (2013.01); G01J 3/021 (2013.01); G01J 3/0289 (2013.01); G01J 3/08 (2013.01); G01J 3/10 (2013.01); G01J 3/4406 (2013.01); G01N 21/636 (2013.01); G02B 21/0044 (2013.01); G02B 21/0076 (2013.01); G02B 21/008 (2013.01); G02B 21/16 (2013.01); G02B 21/18 (2013.01); G02B 21/361 (2013.01); G02B 21/367 (2013.01); G02B 27/1066 (2013.01); G01J 2003/102 (2013.01); G02B 2207/114 (2013.01);
Abstract

We combine linear and nonlinear microscopy modalities. This approach is capable of imaging a sample using the two modalities concurrently, generating time sequences of images of the sample having different properties characteristic of each of the modalities. We can separate light emitted from the sample, generated by either modality, even if the spectrum of light is identical for each modality. The nonlinear microscopy modality allows one to create high resolution volumetric images of the sample. The concurrent imaging of the sample using linear and nonlinear microscopy modalities enables one to correlate a spatial structure and temporal dynamics of the sample acquired in each modality allowing one to create a mapping between images across the modalities. The created mapping can be used to match images of a tissue sample acquired using linear modality in a living organism with high resolution, two- or three-dimensional images obtained using nonlinear microscopy modality.


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