Microfluidic device having specifically designed detection chambers

Abstract

There is provided a microfluidic device () comprising a microfluidic structure having multiple spatially defined cell capturing channels () configured for enabling growth of cells or genetic libraries of cells or cell strains that are capable of producing or secreting compounds. The microfluidic structure of the microfluidic device () further comprises multiple spatially defined detection chambers (A) configured to receive and accommodate target entities. Each of the detection chambers () is configured for fluid connection with at least one of the cell capturing channels (), and has a selective barrier (A) defined between the detection chamber (A) and the respective cell capturing channel or channels () and adapted for allowing flow of at least one of the compounds from the respective cell capturing channel or channels () into the detection chamber (A) enabling the target entities in the detection chamber to be exposed to said at least one compound, while stopping cells or target entities from passing through the selective barrier (A).