The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 2026

Filed:

Apr. 18, 2023
Applicant:

Yunnan Tobacco Quality Supervision and Testing Station, Kunming, CN;

Inventors:

Dan Chen, Kunming, CN;

Yaling Yang, Kunming, CN;

Huimin Deng, Kunming, CN;

Chunqiong Wang, Kunming, CN;

Li Chen, Kunming, CN;

Yifan Cui, Kunming, CN;

Ke Zhang, Kunming, CN;

Shichun Qin, Kunming, CN;

Yuemao Wang, Kunming, CN;

Xiaodong Hu, Kunming, CN;

Xiaodong Yin, Kunming, CN;

Yunfang Zhao, Kunming, CN;

Lijuan Peng, Kunming, CN;

Jie Long, Kunming, CN;

Jieyun Cai, Kunming, CN;

Haowei Sun, Kunming, CN;

Dan Li, Kunming, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/96 (2006.01); B82Y 5/00 (2011.01); C12Q 1/28 (2006.01); G01N 21/78 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
C12N 9/96 (2013.01); B82Y 5/00 (2013.01); C12Q 1/28 (2013.01); G01N 21/78 (2013.01); G01N 33/54346 (2013.01);
Abstract

A nanozyme material and a preparation method thereof are provided. In the preparation method, Prussian blue (PB) is used to regulate the peroxidase activity of Fe,NA/CDs, and then gold nanoparticles are synthesized with Fe,NA/CDs as a reducing agent and a stabilizing agent. The nanozyme material has both a peroxidase activity and a Raman enhancement effect, which can be used for nanozyme-based surface-enhanced Raman spectroscopy (SERS). An embodiment of the present disclosure also provides a method for rapidly detection of a Pbion, which is based on a color change or an additional Raman SERS signal generation caused by nanozyme oxidation. Such sensing signals (colorimetric and SERS analysis) are derived from a catalytic oxidation reaction of ABTS or TMB, while the presence of Pbspecifically inhibits the oxidation, such that the rapid detection of Pbcan be achieved.


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