Tiling light sheet microscope and imaging method of a sample

Abstract

A tiling light sheet microscope and imaging method of a sample. The imaging method includes: generating a first laser beam of a first wavelength range and generating a second laser beam of a second wavelength range; performing a first optical modulation on the first laser beam and performing a second optical modulation on the second laser beam; and then combining the first laser beam and the second laser beam. The combined laser beams are directed onto an illumination path, to generate, by scanning the laser beams, a first excitation light sheet and a second excitation light sheet corresponding to the first wavelength range and the second wavelength range, respectively. The two types of excitation light sheets are combined in a propagation direction of excitation light so as to illuminate the sample. The fluorescences emitted by the sample are collected. In this way, for various biological tissues, at least dual-color simultaneous imaging can be performed, to keep same spatial resolution without increasing image acquisition time.