The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 16, 2026
Filed:
Jul. 29, 2022
Applicant:
Northwestern University, Evanston, IL (US);
Inventors:
Keith E.J. Tyo, Evanston, IL (US);
Catherine Elizabeth Majors, Evanston, IL (US);
Assignee:
Northwestern University, Evanston, IL (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/00 (2006.01); C12Q 1/527 (2006.01); C12Q 1/533 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/004 (2013.01); C12Q 1/527 (2013.01); C12Q 1/533 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01);
Abstract
A method for detecting an analyte is described in which the simultaneously binding of two fusion proteins (i.e., a sandwich assay in solution) is used, bringing two halves of a split enzyme together to produce product, which is detected via a FRET-based biosensor. The method may incorporate an autocatalytic feedback loop that responds to enzymatic product by producing more product to provide ultrasensitive, bistable detection of analyte that is tunable over several orders of magnitude. This system is broadly applicable for protein and small molecule detection.