Dual magnification fluorescence imaging camera

Abstract

A video camera device and system for white light and fluorescence imaging performs imaging for both fluorescence and non-fluorescence light collected from an illuminated scene. A beamsplitter downstream from an optical subsystem separates light a non-fluorescence image into a first imaging channel for and light for a fluorescence image into a second imaging channel. A meniscus lens is positioned downstream from the beamsplitter in one of the first and second imaging channels, and functions to change a magnification such that a magnification of the first image channel is different from a magnification of the second imaging channel. One or more image sensors downstream of the beamsplitter capture the fluorescence and non-fluorescence image light as a fluorescence image and a non-fluorescence image. The fluorescence image has an image size with a smaller area on the sensor or sensors than an image size of the non-fluorescence image.