The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 19, 2026

Filed:

Oct. 15, 2019
Applicant:

Tosoh Corporation, Yamaguchi, JP;

Inventors:

Goshi Kuno, Mie, JP;

Yu Imaizumi, Mie, JP;

Assignee:

TOSOH CORPORATION, Yamaguchi, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12N 11/08 (2020.01);
U.S. Cl.
CPC ...
C12M 25/14 (2013.01); C12M 23/20 (2013.01); C12N 11/08 (2013.01); C12N 2539/10 (2013.01);
Abstract

There are provided a cell culture substrate that enables efficient spheroid formation for cells and can form spheroids having a uniform size and an arbitrary shape at a high cell viability, a method for producing the cell culture substrate, and a method of producing spheroids in which the cell culture substrate is used and a cell viability inside of the spheroids is excellent. A cell culture substrate includes a substrate and a stimulus-responsive polymer coated on the substrate, wherein the stimulus-responsive polymer is a block copolymer having a water-insoluble block segment and a stimulus-responsive block segment, and the cell culture substrate includes the following two regions (A) and (B): (A) an island-shaped region having cell proliferation properties and stimulus responsiveness and having an area of 0.001 to 5 mm; and (B) a region adjacent to the region (A) and having no cell proliferation properties.


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