The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2026

Filed:

Mar. 28, 2025
Applicant:

Allife Medicine (Beijing) Limited, Beijing, CN;

Inventors:

Shixin Gong, Beijing, CN;

Yuchun Gu, Beijing, CN;

Nan Li, Beijing, CN;

Qinqing Peng, Beijing, CN;

Lida Wu, Beijing, CN;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0665 (2013.01); C12N 2500/25 (2013.01); C12N 2500/32 (2013.01); C12N 2500/38 (2013.01); C12N 2500/84 (2013.01); C12N 2501/11 (2013.01); C12N 2501/155 (2013.01); C12N 2501/999 (2013.01); C12N 2506/025 (2013.01);
Abstract

A method for preparing mesenchymal stem cells (MSCs) is provided. The method includes providing expanded potential stem cells or a first cell culture including the expanded potential stem cells; culturing the expanded potential stem cells or the first cell culture in a trophoblast stem cell (TSC) differentiation medium to obtain trophoblast stem cells or a second cell culture including the trophoblast stem cells; culturing the trophoblast stem cells or the second cell culture in a mesenchymal stem cell (MSC) differentiation medium to obtain mesenchymal stem cells or a third cell culture including the mesenchymal stem cells; and passaging the mesenchymal stem cells or the third cell culture in an MSC expansion medium while maintaining characteristics of the mesenchymal stem cells.


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