The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2026

Filed:

May. 22, 2024
Applicant:

University of Pittsburgh—of the Commonwealth System of Higher Education, Pittsburgh, PA (US);

Inventor:

J. Peter Rubin, Pittsburgh, PA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01);
U.S. Cl.
CPC ...
B01L 3/5021 (2013.01); C12M 33/14 (2013.01); C12M 45/05 (2013.01); C12M 45/22 (2013.01); B01L 7/50 (2013.01); B01L 2200/026 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0681 (2013.01);
Abstract

A method of preparing a fat graft includes introducing fat tissue into an internal chamber of a vessel of a cell or tissue isolation, purification, and storage device through a first opening of the vessel. The method also includes introducing an amount of a wash solution effective to wash the fat graft or an amount of a cryoprotectant effective to cryopreserve the fat graft into the internal chamber of the vessel through a second opening of the vessel and mixing the fat tissue with the wash solution or the cryoprotectant. The method further includes either: when wash solution is introduced, centrifuging the device containing the fat graft to separate the fat graft from the wash solution and drawing the wash solution from the first end of the device; or, when cryoprotectant is introduced, cooling the fat graft to a temperature below 0° C.


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