The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 14, 2026

Filed:

Jul. 05, 2018
Applicants:

Kyoto University, Kyoto, JP;

Megakaryon Corporation, Kyoto, JP;

Satake Multimix Corporation, Toda, JP;

Inventors:

Koji Eto, Kyoto, JP;

Sou Nakamura, Kyoto, JP;

Yukitaka Ito, Kyoto, JP;

Tomohiro Shigemori, Kyoto, JP;

Yoshikazu Kato, Toda, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/078 (2010.01); B01F 27/90 (2022.01); C12M 1/00 (2006.01); A61K 35/12 (2015.01); B01F 27/80 (2022.01); B01F 31/00 (2022.01); C12M 1/02 (2006.01); C12M 3/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0644 (2013.01); B01F 27/90 (2022.01); C12M 21/00 (2013.01); C12N 2501/21 (2013.01);
Abstract

Methods of production and apparatuses for production for stably producing healthy and functional platelets, and methods for determining operating conditions of an apparatus for producing platelets are provided. The present invention relates to methods of production comprising a step of culturing megakaryocytes in a platelet production medium C, wherein the step of culturing comprises a step of stirring a medium C in containersusing stirring mechanisms. In one method of production, the step of stirring comprises reciprocating stirring bladesin the stirring mechanismssuch that any of predetermined turbulent energy, shearing stresses, or Kolmogorov scale is produced, in the medium C. In another method of production, the step of stirring comprises pivoting a stirring bladein the stirring mechanismwherein a plurality of stationary membersis placed therearound in a bottom regionof the containersuch that the step of stirring produces turbulence in the medium C. The present invention also relates to apparatuses for production for carrying out the method of production. The present invention also relates to a method for determining operating conditions of an apparatus for producing platelets.


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