The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 07, 2026

Filed:

Jun. 29, 2021
Applicant:

Hoffmann-la Roche Inc., Little Falls, NJ (US);

Inventors:

Tilman Schlothauer, Penzberg, DE;

Christian Spick, Penzberg, DE;

Assignee:

Hoffmann-La Roche Inc., Little Falls, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/557 (2006.01); G01N 33/543 (2006.01);
U.S. Cl.
CPC ...
G01N 33/557 (2013.01); G01N 33/543 (2013.01);
Abstract

Herein is reported a method for determining the dissociation rate constant kof an antibody from its antigen at the dissociation pH-value, wherein the method comprises the steps of immobilizing at a first pH-value the antibody on a solid phase to which the antigen of the antibody has been conjugated; applying a pH-gradient from the first pH-value to the dissociation pH-value and thereafter maintaining the pH-value at said dissociation pH-value; and recording the binding signal during the maintaining of the pH-value and calculating therefrom the dissociation rate constant kof the antibody from its antigen at the dissociation pH-value.


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