The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 24, 2026
Filed:
Apr. 12, 2021
Illumina, Inc., San Diego, CA (US);
Illumina Cambridge Limited, Cambridge, GB;
Eric Vermaas, San Diego, CA (US);
Mahdieh Khosroheidari, San Diego, CA (US);
Angela Kalbande, San Diego, CA (US);
Vincent Peter Smith, Cambridge, GB;
Claire Bevis-Mott, Cambridge, GB;
Jonathan Mark Boutell, Cambridge, GB;
Michael Chesney, Cambridge, GB;
Illumina, Inc., San Diego, CA (US);
Illumina Cambridge Limited, , GB;
Abstract
The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3' ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment and optionally blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment after protective adapters are ligated to target polynucleotides to degrade unincorporated adapters prior to amplification and sequencing, and/or by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.