The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 17, 2026

Filed:

Mar. 30, 2020
Applicants:

Agency for Science, Technology and Research, Singapore, SG;

National University of Singapore, Singapore, SG;

Inventors:

Dong Shin Park, Singapore, SG;

Florent Ginhoux, Singapore, SG;

Mahmoud Pouladi, Singapore, SG;

Jinqiu Zhang, Singapore, SG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/079 (2010.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0622 (2013.01); C12N 5/0697 (2013.01); G01N 33/5082 (2013.01); C12N 2501/22 (2013.01); C12N 2502/081 (2013.01); C12N 2502/1157 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01);
Abstract

A method for generating a microglia-sufficient brain organoid comprising the step of incubating primitive-like macrophage cells with a brain organoid that is between about 15 to about 30 days old in cerebral organoid medium comprising CSF-1 in a low attachment cell culture vessel to generate microglia cells. The present invention also relates to a microglia-sufficient brain organoid obtained by the method as described herein.


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