Gamma delta t cells derived from induced pluripotent stem cells, and production method therefor

Abstract

Provided is a γδ T cell for securing the purity and number of cells sufficient for treatment. Also provided is a method of generating the γδ T cell. More specifically, provided are homogeneous γδ T cells excellent in that the γδ T cells are not affected by exhaustion of the cells. The foregoing is achieved by γδ T cells obtained by subjecting induced pluripotent stem cells (iPS cells) to differentiation induction treatment. Specifically, the foregoing is achieved by γδ T cells generated by subjecting iPS cells having a rearranged γδ TCR gene (γδ TCR-type iPS cells) to differentiation induction treatment. According to the method of generating the γδ T cell of the present invention, there can be provided γδ T cells and a cell population of γδT cells that have an excellent function of having antigen-specific cytotoxic activity in an MHC-unrestricted manner, and that are more homogeneous and have a higher effect than γδ T cells separated from peripheral blood.