The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 10, 2026
Filed:
Mar. 10, 2023
Shandong University, Shandong, CN;
Yanshan University, Hebei, CN;
Chuanzhen Huang, Qinhuangdao, CN;
Zhuang Chen, Jinan, CN;
Hanlian Liu, Jinan, CN;
Peng Yao, Jinan, CN;
Dun Liu, Jinan, CN;
Hongtao Zhu, Jinan, CN;
Bin Zou, Jinan, CN;
Zhen Wang, Qinhuangdao, CN;
Jun Wang, Qinhuangdao, CN;
Longhua Xu, Qinhuangdao, CN;
Shuiquan Huang, Qinhuangdao, CN;
Meina Qu, Qinhuangdao, CN;
Zhengkai Xu, Qinhuangdao, CN;
Minting Wang, Qinhuangdao, CN;
Yabin Guan, Qinhuangdao, CN;
SHANDONG UNIVERSITY, Jinan, CN;
YANSHAN UNIVERSITY, Qinhuangdao, CN;
Abstract
A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.