The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 10, 2026

Filed:

May. 25, 2023
Applicant:

University of Florida Research Foundation, Incorporated, Gainesville, FL (US);

Inventors:

Harry S. Nick, Gainesville, FL (US);

Clive H. Wasserfall, Gainesville, FL (US);

Seth W. Currlin, Gainesville, FL (US);

Jerelyn A. Nick, Gainesville, FL (US);

Andrew J. Nick, Merritt Island, FL (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6428 (2013.01); G01N 2021/6432 (2013.01); G01N 2201/062 (2013.01); G01N 2201/0636 (2013.01);
Abstract

Autofluorescence is the inherent emission of light from cells or tissues that occurs when biological samples are exposed to different wavelengths of excitation light. Tissue autofluorescence is usually ascribed to biological components such as flavins, porphyrins, chlorophyll (in plants), collagen, elastin, red blood cells (RBCs), and lipofuscin. Tissue autofluorescence often presents a significant problem in microscopy, pathology, and surgical applications. Autofluorescence interferes and complicates signal detection in a variety of fluorescence microscopy methods. Tissue autofluorescence reduces the signal detection sensitivity, and in some cases may cause failure in the detection of fluorescent dye signals. Accordingly, there is a need for further improved methods to decrease the autofluorescence intensity from microscopy samples both in research and clinical venues. Described herein is a photobleaching device utilizing a light source tuned to the full spectrum of light to quench autofluorescence of a biological sample.


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