The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 10, 2026

Filed:

Mar. 26, 2020
Applicant:

Medicinal Genomics Corporation, Beverly, MA (US);

Inventors:

Kevin J. Mckernan, Beverly, MA (US);

Yvonne Helbert, Beverly, MA (US);

Heather Ebling, Beverly, MA (US);

Assignee:

MEDICINAL GENOMICS CORPORATION, Beverly, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6851 (2018.01); C12N 9/22 (2006.01); C12Q 1/06 (2006.01); C12R 1/67 (2006.01); C12R 1/68 (2006.01); C12R 1/685 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/06 (2013.01); C12N 9/22 (2013.01); C12Q 1/6851 (2013.01); C12R 2001/67 (2021.05); C12R 2001/68 (2021.05); C12R 2001/685 (2021.05);
Abstract

A method for determining a level of one or more live microbes in a sample of aplant includes (a) introducing a DNase enzyme into a sample including (i)plant matrix derived from lysed cells of aplant and (ii) live microbial cells including microbial genomic DNA, (b) introducing an anionic detergent into the sample, where the anionic detergent is in an amount effective to inactivate the DNase enzyme in the sample and lyse the live microbial cells in the sample, (c) introducing a known quantity of control DNA into the sample, (d) contacting the sample with a plurality of polynucleotide capture agents, (e) isolating the plurality of polynucleotide capture agents, thereby isolating polynucleotides from the microbial genomic DNA and/or control DNA in the sample, and (f) amplifying at least a portion of polynucleotides from the microbial genomic DNA isolated in step (e).


Find Patent Forward Citations

Mckernan, Kevin. (2026). Methods of detecting microbial content in (U.S. Patent No. 12545943). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12545943

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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