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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12529100 B1

PDFFull Text
Date of Patent:
Jan. 20, 2026

Filed:

Oct. 26, 2022

Methods for removal of adaptor dimers from nucleic acid sequencing preparations

Applicant:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

Inventors:

Arkadiusz Bibillo, Cupertino, CA (US);

Randall W. Davis, Pleasanton, CA (US);

Assignee:

Roche Sequencing Solutions, Inc., Pleasanton, CA (US);

Attorney:

Eric Grant Lee

Primary Examiner:

Bradley L Sisson

Int. Cl.
CPC ...
C07H 21/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/6869 (2018.01); C12N 9/00 (2006.01); C12Q 1/683 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6855 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/11002 (2013.01); C12Y 301/11003 (2013.01); C12Y 301/11005 (2013.01); C12Y 301/11006 (2013.01); C12Y 301/15 (2013.01); C12Y 301/21 (2013.01); C12Y 301/22001 (2013.01); C12Y 301/25001 (2013.01);
Abstract

Sequencing adaptors and methods are provided for preparation of polynucleotides for sequencing. The sequencing adaptors contain a portion of a recognition sequence for a methyl-dependent endonuclease. Unwanted adaptor dimers that form during ligation of adaptors to target polynucleotides produce a complete restriction sequence and are cleaved by the endonuclease, followed by exonuclease digestion, thereby removing the dimers.

Find Patent Forward Citations

Bibillo, Arkadiusz. (2026). Methods for removal of adaptor dimers from nucleic acid sequencing preparations (U.S. Patent No. 12529100). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12529100

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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