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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12529038 B1

PDFFull Text
Date of Patent:
Jan. 20, 2026

Filed:

Dec. 22, 2022

Applying optogenetic amplifier circuits for multi-phase light controlled microbial fermentations

Applicant:

The Trustees of Princeton University, Princeton, NJ (US);

Inventors:

Jose L. Avalos, Princeton, NJ (US);

Jared E. Toettcher, Princeton, NJ (US);

Evan M. Zhao, Clarence Center, NY (US);

Makoto A. Lalwani, San Jose, CA (US);

Assignee:

THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US);

Attorney:

Meagher Emanuel Laks Goldberg & Liao, LLP

Primary Examiner:

Yong D Pak

Int. Cl.
CPC ...
C12N 9/04 (2006.01); C12N 1/16 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 15/63 (2006.01); C12N 15/81 (2006.01); C12P 7/00 (2006.01); C12P 7/56 (2006.01); C12P 17/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0006 (2013.01); C12N 1/16 (2013.01); C12N 9/1022 (2013.01); C12N 9/88 (2013.01); C12N 15/635 (2013.01); C12N 15/81 (2013.01); C12P 7/56 (2013.01); C12P 17/06 (2013.01); C12Y 101/01027 (2013.01); C12Y 202/01006 (2013.01); C12Y 401/01005 (2013.01); C12Y 403/01023 (2013.01); C12Y 403/01024 (2013.01); C12N 2529/10 (2013.01); C12N 2800/102 (2013.01); C12N 2830/002 (2013.01);
Abstract

Disclosed is a technique for constructing optogenetic amplifier and inverter circuits utilizing transcriptional activator/repressor pairs, in which expression of the transcriptional activator or repressor, respectively, is controlled by light-controlled transcription factors. This system is demonstrated utilizing the quinic acid regulon system from, or Q System, a transcriptional activator/repressor system. This is also demonstrated utilizing the galactose regulon from, or GAL System. Such optogenetic amplifier circuits enable multi-phase microbial fermentations, in which different light schedules are applied in each phase to dynamically control different metabolic pathways for the production of proteins, fuels or chemicals. The orthogonal nature of the Q and GAL systems enable the co-expression of amplifier and inverter circuits to simultaneously amplify and invert the response of light-controlled transcriptional controls over different sets of genes in the same cell.

Find Patent Forward Citations

Avalos, Jose. (2026). Applying optogenetic amplifier circuits for multi-phase light controlled microbial fermentations (U.S. Patent No. 12529038). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12529038

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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