The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 13, 2026

Filed:

Sep. 19, 2020
Applicant:

Janssen Biotech, Inc., Horsham, PA (US);

Inventor:

Harsha Gunawardena, Lafayette Hill, PA (US);

Assignee:

JANSSEN BIOTECH, INC., Horsham, PA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); C07K 1/13 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); C07K 1/13 (2013.01); G01N 2458/15 (2013.01);
Abstract

Methods of analyzing a drug polypeptide conjugate using tandem mass spectrometry involving dual tandem mass tags (TMTs) are described. Provided herein is an integrated method using TMTs to obtain three analytical measurements, termed 'triple play', which enables identification of the drug occupancy, normalization between multiple samples and triggering of additional MS/MS to identify and localize conjugation site(s) of the payload. Also described is a method of multiplexing conjugation reactions in a single run with TMT labeling for enhanced throughput capability, while maintaining the same sensitivity with current mass spectrometry instrumentation.


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