The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 30, 2025

Filed:

Jan. 07, 2022
Applicant:

Pioneer Hi-bred International, Inc., Johnston, IA (US);

Inventors:

Ajith Anand, Davis, CA (US);

Maren L Arling, Des Moines, IA (US);

Alexandre Da Silva Conceicao, Wilmington, DE (US);

William James Gordon-Kamm, Urbandale, IA (US);

Craig Hastings, Perry, IA (US);

George J Hoerster, Des Moines, IA (US);

Todd J Jones, Johnston, IA (US);

Theodore M Klein, Wilmington, DE (US);

Carlos M La Rota, Johnston, IA (US);

Keith S Lowe, Johnston, IA (US);

Shiv Bahadur Tiwari, San Jose, CA (US);

Ning Wang, Johnston, IA (US);

Xinli Emily Wu, Johnston, IA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); A01H 4/00 (2006.01); C07K 14/415 (2006.01);
U.S. Cl.
CPC ...
A01H 4/008 (2013.01); C12N 15/8201 (2013.01); C07K 14/415 (2013.01); C12N 15/8213 (2013.01); C12N 15/8216 (2013.01);
Abstract

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.


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