The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 23, 2025

Filed:

May. 17, 2021
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

Melissa C. Skala, Middleton, WI (US);

Kayvan Samimi, Madison, WI (US);

Emmanuel Contreras-Guzman, Madison, WI (US);

Alexandra Jule Walsh, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12N 5/00 (2006.01); C12N 5/0783 (2010.01); G01N 15/10 (2024.01); G01N 15/14 (2006.01); G01N 15/149 (2024.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); C12N 5/0087 (2013.01); C12N 5/0636 (2013.01); G01N 15/1459 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/143 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0864 (2013.01); C12N 2521/00 (2013.01); C12N 2529/10 (2013.01); G01N 2015/1006 (2013.01); G01N 15/149 (2024.01);
Abstract

Systems and methods for classifying T cells by activation state are disclosed. The system includes a cell analysis pathway, a time-resolved autofluorescence decay spectrometer, a processor, and a non-transitory computer-readable memory. The memory is accessible to the processor and has stored thereon instructions. The instructions, when executed by the processor, cause the processor to: a) receive the time-resolved autofluorescence decay signal; b) compute at least a first phasor coordinate at a first frequency and a second phasor coordinate at a second frequency from the time-resolved autofluorescence decay signal, wherein the first and second frequency are different; and c) compute an activation prediction for the T cell using at least the first phasor coordinate and the second phasor coordinate.


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