The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 16, 2025

Filed:

Jul. 28, 2022
Applicant:

Shimadzu Corporation, Kyoto, JP;

Inventor:

Natsuki Iwata, Kyoto, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 30/88 (2006.01); G01N 33/68 (2006.01); G01N 35/10 (2006.01); G01N 30/02 (2006.01);
U.S. Cl.
CPC ...
G01N 30/88 (2013.01); G01N 33/6812 (2013.01); G01N 35/1016 (2013.01); G01N 2030/027 (2013.01); G01N 2030/8804 (2013.01); G01N 2030/8818 (2013.01);
Abstract

A method for analyzing amino acids by liquid chromatography, in which a sample containing a plurality of kinds of amino acids is derivatized with a derivatization reagent, and the obtained derivatized sample is circulated on a column together with a mobile phase. Two or more kinds of derivatized samples are prepared using two or more kinds of derivatization reagents. Different analytical conditions in which mixing ratio of the plurality of the mobile phases is changed with a passage of time are set for each kind of the derivatization reagent. A solvent mixing ratio in the mobile phase being the mixed solvent system is set for the each kind of the derivatization reagent. The two or more kinds of derivatized samples are analyzed by automatically switching between the different analytical conditions and the solvent mixing ratio to separate and quantify derivatized L-amino acids and derivatized D-amino acids.


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